Difference Between Blood Agar And Chocolate Agar

Difference between Blood Agar and Chocolate Agar

Hi everyone!

I’ll tell you in short about Nutrient agar first

It is a simple basal medium used for growth of common pathogens

It constitutes peptone water, meat extract and agar

Difference between Blood Agar and Chocolate Agar

What is common between Blood agar and Chocolate agar?

Both are enriched media

Used for the growth of Gram positive cocci and fastidious organisms like Neisseria & Haemophilus species

Can be used to indicate hemolysis

What are fastidious organisms?

They require specialized environments due to complex nutritional requirement

What is the difference between Blood agar and Chocolate agar?

The difference lies in how the media are made

How is Blood agar made?

Nutrient agar is sterilized by autoclave, cooled to 50°C and sterile sheep blood (5-10%) is added gradually and poured into plates

How is Chocolate agar made?

Nutrient agar is sterilized by autoclave, cooled to 75-80°C and sterile sheep blood (5-10%) is added gradually and poured into plates

How is the difference in temperature significant?

Certain organisms such as Haemophilus species require V factor for growth (complex nutritional requirement)

Factor V is present in blood but it is present inside the red blood cells (RBC)

These organisms can not utilize V factor which is trapped inside the RBC’s in Blood agar

When Blood agar is heated to 80-90°C for a few minutes (boiled blood agar), the V factor is released from within the erythrocytes and made available to the organism for utilization

Some strains of Neisseriae and Diphtheroids require V factor too

That’s why these media are superior to plain Blood agar for growing organisms requiring V factor

What is V fatcor?

V fatcor is a coenzyme, Nicotinamide Adenine Dinucleotide (NAD) or Nicotinamide Adenine Dinucleotide Phosphate (NADP) which acts as a hydrogen acceptor in the metabolism of cell

So to conclude, Chocolate agar is a type of blood agar in which the blood cells have been lysed by heating for growing fastidious organisms :)

Did you know?

Modified Thayer Martin is a type of chocolate agar which contains antibiotics (Vancomycin, Nystatin & Colistin) to suppress growth of other bacteria and promote growth of  N gonorrhoeae

*phew* That’s all for today!

The image is my first photoshop work on the site, hope you like it =D

Just to remind you guys, Chocolate agar contains no chocolate, they simply named it after the yummy color :P

-IkaN

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6 years ago
Sketchy Micro To Do List: 
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Sketchy Micro To Do List: 

• 01 - Gram Positive Cocci o 1.1 - Staph Aureus (11:03) o 1.2 - Staph Epidermidis (6:54) o 1.3 - Strep pyogenes (Group A Strep) (14:30) o 1.4 - Strep agalactiae (Group B Strep) (5:23) o 1.5 - Strep. pneumoniae Strep viridans (9:17) o 1.6 - Enterococcus (4:06) • 02 - Gram Positive Bacilli o 2.1 - Bacillus anthracis and Bacillus cereus (9:50) o 2.2 - Clostridium tetani (6:42) o 2.3 - Clostridium botulinum (7:35) o 2.4 - Clostridium difficile (8:17) o 2.5 - Clostridium perfringens (5:31) o 2.6 - Corynebacterium diphtheriae (6:49) o 2.7 - Listeria monocytonegenes (4:04) • 03 - Gram-Positive Branching Filamentous Rods o 3.1 - Actinomyces (3:01) o 3.2 - Nocardia (6:50) • 04 - Gram-Negative Cocci o 4.1 - Neisseria species overview (5:07) o 4.2 - Neisseria meningitidis (8:59) o 4.3 - Neisseria gonorrheae (7:33) • 05 - Gram-Negative Bacilli - Enteric tract o 5.1 - Klebsiella, Enterobacter, Serratia (7:49) o 5.10 - Proteus mirabilis (2:54) o 5.2 - Salmonella (5:51) o 5.3 - Shigella (6:26) o 5.4 - Escherichia coli (ETEC, EHEC) (8:51) o 5.5 - Yersinia enterocolitica (7:54) o 5.6 - Campylobacter (5:30) o 5.7 - Vibrio (5:45) o 5.8 - Helicobacter (5:23) o 5.9 - Pseudomonas (9:59) • 06 - Gram-Negative Bacilli - Respiratory tract o 6.1 - Bordatella pertussis (7:39) o 6.2 - Haemophilus influenzae (8:46) o 6.3 - Legionella (7:26)

• 07 - Gram-Negative Bacilli - Zoonotics o 7.1 - Bartonella henselae (4:15) o 7.2 - Brucella (4:41) o 7.3 - Francisella tularensis (3:50) o 7.4 - Pasteurella multocida (3:55) • 08 - Mycobacteria o 8.1 - Mycobacterium tuberculosis (16:35) o 8.2 - Mycobacterium leprae (9:17) • 09 - Spirochetes o 9.1 - Borrelia (8:16) o 9.2 - Leptospirosis (4:18) o 9.3 - Treponema Pallidum (12:52) • 10 - Gram-Indeterminate Bacteria o 10.1 - Chlamydia (15:08) o 10.2 - Coxiella burnetii (4:57) o 10.3 - Gardnerella vaginalis (5:32) o 10.4 - Mycoplasma pneumoniae (5:57) o 10.5 - Rickettsia species overview (3:34) o 10.6 - Rickettsia prowazekii (4:11) o 10.7 - Rickettsia rickettsii (4:00) • Fungi • 1 - Systemic Mycoses o 1.1 - Histoplasmosis (9:38) o 1.2 - Blastomycosis (6:09) o 1.3 - Coccidioidomycosis (7:26) o 1.4 - Paracoccidioidomycosis (4:55) • 2 - Cutaneous Mycoses o 2.1 - Malassezia furfur :0Pityriasis Versicolor (5:10) o 2.2 - Dermatophytes (6:01) o 2.3 - Sporothrix schenckii (4:24) • 3 - Opportunistic Fungal Infections o 3.1 - Candida albicans (12:49) o 3.2 - Aspergillus fumigatus (10:51) o 3.3 - Cryptococcus neoformans (9:00) o 3.4 - Mucormycosis (6:17) o 3.5 - Pneumocystis pneumonia (5:49)

• Parasites • 1 - Protozoa of the Intestinal Tract o 1.1 - Giardia (5:19) o 1.2 - Entamoeba histolytica (7:55) o 1.3 - Cryptosporidium (4:31) • 2 - Protozoa of the CNS o 2.1 - Toxoplasmosis (10:21) o 2.2 - Trypanosoma brucei (4:45) o 2.3 - Naegleria fowleri (6:01) • 3 - Protozoa of the Blood o 3.1 - Trypanosoma cruzi (5:43) o 3.2 - Babesia (6:59) o 3.3 - Plasmodium species (13:57) o 3.4 - Leishmaniasis (5:47) • 4 - Protozoa - Other Tissue o 4.1 - Trichomoniasis (5:37) • 5 - Helminths - Nematodes o 5.1 - Intestinal nematodes (13:09) o 5.2 - Tissue Nematodes (11:29) • 6 - Helminths - Trematodes and Cestodes o 6.1 - Cestodes (10:20) o 6.2 - Trematodes (10:56)

• Viruses • 1 - RNA Viruses - Positive Sense o 1.1 - Picornavirus Overview (10:47) o 1.2 - (Pico) Poliovirus (7:18) o 1.3 - (Pico) Coxsackievirus (5:10) o 1.4 - (Pico) Rhinovirus (4:45) o 1.5 - (Pico) Hepatitis A (7:00) o 1.6 - (Norwalk) Calicivirus (5:26) o 1.7 - Flavivirus (8:16) o 1.8 - Hepatitis C Virus (10:24) o 1.9 - Togavirus (11:20) o 1.10 - Coronavirus (3:32) o 1.11 - HIV (16:24) • 2 - RNA Viruses - Negative Sense o 2.1 - Orthomyxovirus (18:23) o 2.2 - Paramyxovirus (15:57) o 2.3 - Rhabdovirus (9:02) o 2.4 - Filovirus (4:54) o 2.5 - Bunyavirus (5:41) o 2.6 - Arenavirus (5:15) o 2.7 - Reovirus (7:15) • 3 - DNA Viruses o 3.1 - HSV-1 and HSV-2 (11:15) o 3.10 - Adenovirus (4:52) o 3.11 - Poxvirus (8:26) o 3.12 - Hepatitis B Virus (18:38) o 3.2 - EBV (13:24) o 3.3 - CMV (11:51) o 3.4 - VZV (11:45) o 3.5 - HHV-6 (5:12) o 3.6 - HHV-8 (7:10) o 3.7 - Polyomavirus JC & BK (6:31) o 3.8 - Papillomavirus (13:47) o 3.9 - Parvovirus (7:07)

6 years ago

I need tips on taking textbook notes please! I always get bored reading and then I end up just writing down bolded words with no context (which is really bad I know) any tips to help take more efficient textbook notes without having to spend hours (because I have 3 content heavy classes that I need to take notes for)

Hi there! I know this post may feel basic, but I feel like you’re struggling with basic skills like summarising a topic and writing things in your own words, so I tried my best to explain things in a step-by-step action-oriented list so that you can stand on your own ‘feet’ when you’re studying :)

The Basics of Studying From a Textbook

1. The textbook may be boring, but you’ve still got to read it. 

Unfortunately, high school and university isn’t a walk in the park :( I know, some days it’s hard, but you’ve still got to put in at least a modicum of hard work if you want to do well. 

Sometimes, the studyblr community perpetuates the idea that there’s some sort of “secret” to being a straight-As, 4.0GPA, HD WAM student, but there’s only studying techniques to make things easier. You’re still going to need a basic level of motivation, discipline, time management, organisation and study in order to do well. 

So even though the textbook is boring, you’re either going to have to convince yourself that the topic is interesting, or pull up your socks, buckle down, and actually read it. 

2. Reading speed is a limiting factor on how fast you can take notes. 

Try reading the textbook without taking any notes and time how long it takes you. That’s going to be the minimum time it takes, so if doing all the reading for 3 content heavy courses takes at least an hour or two, then reading and taking notes is going to be much more than that (roughly more than double the time), so you can’t speed it up any faster than your base reading speed. 

I recommend trying speed reading techniques, but that would take a whole separate post, so I won’t go into that here. 

3. Learn how to summarise a topic. 

At the moment it seems like you’re struggling and not really understanding how to summarise topics, and just relying on the textbook to tell you what’s important. 

To summarise something, you’ll need to write down the key points (the most important information) from something that you read in the fewest number of words reasonable. I’m sure you already knew that, but you must not be putting it into practice because of what you’re telling me in your ask. 

4. Write things in your own words. 

Again, once you learn how to summarise, writing it in your own words will solidify that information in your brain. Explain the topic in layman’s terms to a family member, which forces you to use your own words. If you live alone, try a stuffed toy or rubber duck. 

5. Start off by annotating your class notes. 

I think initially you should start off with annotating just until you learn what information is important and what’s not. Class notes usually have 80% of the important information in brief points, and the textbook colours in the rest. 

6. Use the “gummy bears” method. 

I know it’s elementary, but you seemed to be having trouble with discipline and boredom, so place gummy bears on every paragraph as a crutch to get yourself to finish reading everything. Once you train yourself to do things that you don’t want to do, then you won’t have to use any cheap tricks, as you’ll be disciplined enough to read pages and pages without needing constant “carrot on a stick” rewards. 

Once you’ve done those, here are my masterposts for content heavy courses:

Studying Content Heavy Courses

Use my Unique Automated Study Planner Printable which uses spaced repetition to make you remember more strategically!

Content Heavy Courses Study Guide - biology used as an example

Self Studying Advice - when you have to study a lot by yourself

Staying Productive No Matter How Much Time You Have

The Blank Paper Method - for rote learning lots of information

Part 11 Adapting to Uni Study - university basically mandates studying a large amount of info in a short period, so you’ll find this post useful for balancing 6 heavy courses!

Part 12 How to Study From Textbooks in Uni

Hope that helps!!

Follow optomstudies for daily original posts and study masterposts! Links: all originals + langblr posts + 15-part college 101 series + web directory!

6 years ago

Remember to use your sponge to replace any bacteria on your dishes accidentally removed by the act of eating.

Microbiology professor (via scienceprofessorquotes)

6 years ago
Be Patient, Good Things Are Coming Your Way :)

be patient, good things are coming your way :)

6 years ago
Me This Week

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6 years ago
Motivation Can Be Experienced differently for Each Of Us. While Some People Just Get This Kind Of Impulsive

Motivation can be experienced differently for each of us. While some people just get this kind of impulsive motivation, others develop their motivation through actions.

@masterposts @studymasterposts

I would like to show you why it is wrong to wait for your motivation and why you should just do something instead - it doesn’t matter how big your action is.

How to gain motivation

OK, so just like I told you above there exists a concept of just doing some random shit. That means that you start small even though you know that you should do more.

You need to write an essay in two days but you’re spending all the time watching a cat jumping from a window? (if you followed the link - yeah, it’s funny but please continue reading my bullshit lol)

The solution to your stressful task is to start doing something even if it doesn’t seem big or challenging. (In our example with the essay this would mean, starting the writing program/ writing “Outline for my essay” on a paper/ starting a draft as small as possible etc.)

You need to clean your room? Search the smallest task and do this one: put your old pizza that’s laying on the floor in the trash can and your underwear where it belongs - you don’t want your dog to eat it, right?

You want to get fit and start working out? Maybe you had an impulse of motivation one week ago, but now you’re laying on the couch like the cutest panda ever, eating sweets and asking yourself where your motivation went.

You already have an idea what you need to do now, right? Just do one push-up/ lunge/ crunch whatever.

It doesn’t matter how small your action is, but if you experience the feeling of this small success you’ll keep going.

How to stay motivated (Part 1)

Now that you’re finally motivated (doesn’t matter how), you really need to stick on that workflow or whatever you’re trying to achieve. One of the simplest things to maintain your motivation is to think of why you started. Here are some examples:

I know that I’m able to get this shit done.

I want to prove that I can do more than I thought.

I want to show everybody that I’m taking my responsibilities seriously.

I need to pass that exam.

Failing is worse than studying.

etc. (the reason needs to come from your heart - if it isn’t true, it won’t work)

Another way (which is pretty simple and works pretty good for most of the people) is the chain system: 

Draw 31 boxes (for one month) and tick one each day after completing your task. This method is pretty common because it is pretty simple and works for no matter how much time you want to do your habit. (The lovely @emmastudies has created a lot of wonderful printables, maybe you find more details there for study tracking.) After keeping up with the growing chain, you’ll be thinking twice before interrupting the chain and stopping your chain.

Part 2 will be posted on Friday, 06.01.

6 years ago
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Immuno
Immuno
Immuno
Immuno
Immuno
Immuno

Immuno

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You can get the Sticker here: https://www.redbubble.com/people/histrionicole/works/29980305-lymph-node-drainage?asc=u&p=sticker

6 years ago
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Microbio Lab Work 📖
Microbio Lab Work 📖
Microbio Lab Work 📖

Microbio lab work 📖

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