Tularemia

volutin granules are an intracytoplasmic storage form of complexed inorganic polyphosphate, the production of which is used as one of the identifying criteria when attempting to isolate Corynebacterium diphtheriae on Löffler’s medium….look like chines letters…as given below

Different anatomy notes form this semester Supplies used (not all at once, I mix and match): Faber-Castell Coloured Pencils (48 Pack) - https://amzn.to/2Kd1mUy Staedtler Triplus Fineliners - http://amzn.to/2pghonI Stabilo Point 88 Fineliner - https://amzn.to/2qU8fC9 Sharpie Pens - https://amzn.to/2HTRmP2 Uni Pin 0.1 Fineliner - https://amzn.to/2HmXp1z Crayola Supertips - https://amzn.to/2HVW1jr Bic Ballpoint Pen - https://amzn.to/2HmCjk0 Stabilo Swing Cool Highlighters - https://amzn.to/2HKxPTu

• Microbiology cheat sheets for the comprehensive final. We were allowed two pages, front and back. I ended up getting a 95%! •

ANTIBIOTICS CHEAT SHEET :)

Also, REMEMBER!!!!

* Sulfonamides compete for albumin with:

Bilirrubin: given in 2°,3°T, high risk or indirect hyperBb and kernicterus in premies

Warfarin: increases toxicity: bleeding

* Beta-lactamase (penicinillase) Suceptible:

Natural Penicillins (G, V, F, K)

Aminopenicillins (Amoxicillin, Ampicillin)

Antipseudomonal Penicillins (Ticarcillin, Piperacillin)

* Beta-lactamase (penicinillase) Resistant:

Oxacillin, Nafcillin, Dicloxacillin

3°G, 4°G Cephalosporins

Carbapenems 

Monobactams

Beta-lactamase inhibitors

* Penicillins enhanced with:

Clavulanic acid & Sulbactam (both are suicide inhibitors, they inhibit beta-lactamase)

Aminoglycosides (against enterococcus and psedomonas)

* Aminoglycosides enhanced with Aztreonam

* Penicillins: renal clearance EXCEPT Oxacillin & Nafcillin (bile)

* Cephalosporines: renal clearance EXCEPT Cefoperazone & Cefrtriaxone (bile)

* Both inhibited by Probenecid during tubular secretion.

* 2°G Cephalosporines: none cross BBB except Cefuroxime

* 3°G Cephalosporines: all cross BBB except Cefoperazone bc is highly highly lipid soluble, so is protein bound in plasma, therefore it doesn’t cross BBB.

* Cephalosporines are "LAME“ bc they  do not cover this organisms 

L  isteria monocytogenes

A  typicals (Mycoplasma, Chlamydia)

M RSA (except Ceftaroline, 5°G)

E  nterococci

* Disulfiram-like effect: Cefotetan & Cefoperazone (mnemonic)

* Cefoperanzone: all the exceptions!!!

All 3°G cephalosporins cross the BBB except Cefoperazone.

All cephalosporins are renal cleared, except Cefoperazone.

Disulfiram-like effect

* Against Pseudomonas:

3°G Cef taz idime (taz taz taz taz)

4°G Cefepime, Cefpirome (not available in the USA)

Antipseudomonal penicillins

Aminoglycosides (synergy with beta-lactams)

Aztreonam (pseudomonal sepsis)

* Covers MRSA: Ceftaroline (rhymes w/ Caroline, Caroline the 5°G Ceph), Vancomycin, Daptomycin, Linezolid, Tigecycline.

* Covers VRSA: Linezolid, Dalfopristin/Quinupristin

* Aminoglycosides: decrease release of ACh in synapse and act as a Neuromuscular blocker, this is why it enhances effects of muscle relaxants.

* DEMECLOCYCLINE: tetracycline that’s not used as an AB, it is used as tx of SIADH to cause Nephrogenic Diabetes Insipidus (inhibits the V2 receptor in collecting ducts)

* Phototoxicity: Q ue S T  ion?

Q uinolones

Sulfonamides

T etracyclines

* p450 inhibitors: Cloramphenicol, Macrolides (except Azithromycin), Sulfonamides

* Macrolides SE: Motilin stimulation, QT prolongation, reversible deafness, eosinophilia, cholestatic hepatitis

* Bactericidal: beta-lactams (penicillins, cephalosporins, monobactams, carbapenems), aminoglycosides, fluorquinolones, metronidazole.

* Baceriostatic: tetracyclins, streptogramins, chloramphenicol, lincosamides, oxazolidonones, macrolides, sulfonamides, DHFR inhibitors.

* Pseudomembranous colitis: Ampicillin, Amoxicillin, Clindamycin, Lincomycin.

* QT prolongation: macrolides, sometimes fluoroquinolones

Archaea

Archaeans are single-celled and join bacteria to make up the Prokaryotes.  The Archaea classification is a very recent discovery, due to the similarities in appearance and behaviour to bacteria they weren’t separated until the late 1970′s. They mostly live in extreme environments and can be sub grouped: 

Methanogens - produce methane gas as a waste product of their “digestion,” or process of making energy.

Halophiles - live in salty environments.

Thermophiles - live at extremely hot temperatures.

Psychrophiles — those that live at unusually cold temperatures.

Like bacteria, archaea lack a true nucleus. Both bacteria and archaea usually have one DNA molecule suspended in the cell’s cytoplasm contained within a cell membrane. Most, but not all, have a tough, rigid outer cell wall.

use a variety of substances for energy, including hydrogen gas, carbon dioxide and sulfur. 

many archaea thrive in conditions mimicking those found more than 3.5 billion years ago. [eg oceans that regularly reached boiling point — an extreme condition not unlike the hydrothermal vents and sulfuric waters where archaea are found today]

Flashcards  with quizlet!

For more: instagram: https://www.instagram.com/studydiaryofamedstudent/

Some general resources:

Chemistry Glossary

Chemistry Exam Survival Guide

Toolbox – interactive graphing, tables, and calculators

Make virtual chemistry models

Interactive periodic table

Another site for making virtual chemistry models

Virtual labs – covers stoichiometry, thermochemistry, eq1uilibrium, acid base chemistry, solubility, oxidation/reduction and electrochemistry, analytical chemistry/lab techniques

Concept tests

Chemistry Science Fair Project Ideas

OChem Reaction Bank

Interactive chem simulations

Chemical calculations

The Chem Blog

Molecule of the day

Free chemistry drawing software

Laboratory Safety - Laboratory safety for the chemistry classroom

Periodic Table of Videos - Brady Haran

On this day in chemistry… - a history of chemistry

The faces of chemistry

Experimentation hub -  explore and enjoy our experiments to increase engagement in scientific investigation, develop new skills and enhance your knowledge.

Understanding journals - including reading articles, referencing, and example articles.

Resources for specific topics:

Stochiometry – the mole, molarity and density, reaction stoichiometry and limiting reagents, empirical formula and mixtures, gravimetric analysis

Themochemistry – energy and enthalpy, entropy

Kinetics – phenomenological and mechanistic kinetics

Equilibrium – LeChatlier’s principle, progress of reaction, equilibrium calculations, common ion effect

Acid base chemistry – strong acid and bases, weak acids and bases, buffer solutions, acid/base titrations

Solubility – solubility product, solubility and PH, common ion effect

Oxidation/Reduction and Electrochemistry – standard reduction potentials, galvanic cells

Analytical chemistry/ Lab techniques – reaction stoichiometry and limiting reagents, acid/base titrations, redox titrations, gravimetric analysis, UC/Vis spectroscopy

Physical chemistry – quantum mechanics, spectroscopy

Properties of solutions – intermolecular forces, colligative properties

Textbooks:

Chemistry Virtual Textbooks, Stephen Lower

Organic Chemistry, Tim Soderberg

Organic Chemistry I, George Mhehe

Environmental Chemistry, Dejene Tessema

Virtual Organic Chemistry

Industrial Chemistry, Helen Njenga

Inorganic Chemistry, Chrispin Kowenje

Physical Chemistry I, Onesmus Munyaki

General Chemistry, Principles, Patterns and Applications

Chemistry Books - a variety of chemistry textbooks

Chemistry Tutorials/Guides:

Atoms, Molecules, and Ions

Chemical reactions and stoichiometry

Electronic structure of atoms

Periodic table

Chemical bonds

Gases and kinetic molecular theory

State of matter and intermolecular forces

Chemical equilibrium

Acids and bases

Acid base equilibria and solubility equilibria

Thermodynamics

Redox reactions and electrochemistry

Kinetics

Nuclear chemistry

Organic Chemistry Tutorials/Guidelines:

Structure and bonding

Dot structures

Hybridization

Bond-line structures

Electronegativity

Resonance and acid base chemistry

Counting electrons

Resonance structures

Organic acid-base chemistry

Alkanes, cycloalkanes and functional groups

Naming alkanes

Naming alkanes, cycloalkanes, and bicyclic compounds

Conformations of alkanes

Conformations of cycloalkanes

Functional groups

Stereochemistry

Chirality

Enantiomers

Stereoisomeric relationships

Subsituation and elimination reactions

Free radical reaction

Sn1 vs Sn2

Nucleophilicity and basicity

Elimination reactions

Sn1/Sn2/E1/E2

Sn1 and Sn2

Alkenes and alkynes

Naming alkenes

Alkene reactions

Alkene nomenclature

Alkene reactions

Naming and preparing alkynes

Alkyne reactions

Alcohols, ethers, epoxides, sulphides

Alcohol nomenclature and properties

Synthesis of alcohols

Reactions of alcohols

Nomenclature and properties of ethers

Synthesis and cleavage of ethers

Nomenclature and preparation of epoxides

Conjugation, Diels-Alder, and MO theory

Addition reactions of conjugated dienes

Diels-Alder reaction

Molecular orbital theory

Aromatic compounds

Naming benzene derivatives

Reactions of benzene

Aromatic stability

Electrophilic aromatic substitution

Directing effects

Other reactions and synthesis

Aldehydes and ketones

Introduction to aldehydes and ketones

Reactions of aldehydes and ketones

Carboxylic acids and derivatives

Naming carboxylic acids

Formation of carboxylic acid derivatives

Nomenclature and reactions of carboxylic acids

Nomenclature and reactions of carboxylic acid derivatives

Alpha carbon chemistry

Formation of enolate anions

Aldol condensations

Amines

Naming amines

Spectroscopy

Infrared Spectroscopy

UV/Vis Spectroscopy

proton NMR

Careers:

A future in Chemistry

What can I do with my chemistry degree?

Chemistry Careers - American Chemical Society

What to do with a degree in chemistry - The Guardian

MORE MIXED MNEMONICS

Penicillin

Penicillin is a widely used antibiotic prescribed to treat staphylococci and streptococci bacterial infections. 

beta-lactam family 

Gram-positive bacteria = thick cell walls containing high levels of peptidoglycan

gram-negative bacteria = thinner cell walls with low levels of peptidoglycan and surrounded by a lipopolysaccharide (LPS) layer that prevents antibiotic entry 

penicillin is most effective against gram-positive bacteria where DD-transpeptidase activity is highest.

Examples of penicillins include:

amoxicillin

ampicillin

bacampicillin

oxacillin

penicillin

Mechanism(s)

Penicillin inhibits the bacterial enzyme transpeptidase, responsible for catalysing the final peptidoglycan crosslinking stage of bacterial cell wall synthesis.

Cells wall is weakened and cells swell as water enters and then burst (lysis)

Becomes permanently covalently bonded to the enzymes’s active site (irreversible)

Alternative theory: penicillin mimics D-Ala D-Ala

Or may act as an umbrella inhibitor

Resistance

production of beta-lactamase - destroys the beta-lactam ring of penicillin and makes it ineffective (eg Staphylococcus aureus - most are now resistant)

In response, synthetic penicillin that is resistant to beta-lactamase is in use including egdicloxacillin, oxacillin, nafcillin, and methicillin. 

Some is resistant to methicillin - methicillin-resistant Staphylococcus aureus (MRSA).  

Demonstrating blanket resistance to all beta-lactam antibiotics -extremely serious health risk.