CRISP(ie)R News Than Anything!

CRISP(ie)R news than anything!

Enzyme tweak boosts precision of CRISPR genome edits
Engineered enzyme drives genome-editing errors below detection limit.

A powerful technique for editing genomes is now more precise. By tweaking an enzyme, researchers have reduced the error rate for the technique, known as CRISPR–Cas9 — in some cases to undetectable levels, they report on 6 January in Nature1.

Researchers use CRISPR–Cas9 to make precise changes to genomes that remove or edit a faulty gene. It has worked on nearly every creature on which they have tested it, including human embryos.

The technique relies on an enzyme called Cas9, that uses a ‘guide RNA’ molecule to home in on its target DNA. Cas9 cuts the DNA at that site, and the cell’s natural DNA repair machinery then takes over to mend the cut — deleting a short fragment of DNA or stitching in a new sequence in the process.

But the technology is not infallible: sometimes the Cas9 enzyme creates unwanted mutations. As CRISPR inches out of the laboratory and towards the clinic — with debates raging overwhether it should be deployed in embryos — researchers have pushed to reduce the error rate.

The latest study moves the field closer to that goal, says lead author Keith Joung, a pathologist at Massachusetts General Hospital in Boston. “This is a significant move forward,” he says. “We can very much reduce the probability of off-targets.”

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More Posts from Redplanet44 and Others

5 years ago
Origami-inspired Materials Could Soften The Blow For Reusable Spacecraft

Origami-inspired materials could soften the blow for reusable spacecraft

Space vehicles like SpaceX’s Falcon 9 are designed to be reusable. But this means that, like Olympic gymnasts hoping for a gold medal, they have to stick their landings.

Landing is stressful on a rocket’s legs because they must handle the force from the impact with the landing pad. One way to combat this is to build legs out of materials that absorb some of the force and soften the blow.

University of Washington researchers have developed a novel solution to help reduce impact forces – for potential applications in spacecraft, cars and beyond. Inspired by the paper folding art of origami, the team created a paper model of a metamaterial that uses “folding creases” to soften impact forces and instead promote forces that relax stresses in the chain. The team published its results May 24 in Science Advances.

“If you were wearing a football helmet made of this material and something hit the helmet, you’d never feel that hit on your head. By the time the energy reaches you, it’s no longer pushing. It’s pulling,” said corresponding author Jinkyu Yang, a UW associate professor of aeronautics and astronautics.

Read more.

6 years ago
This Brainless Slime Learns And Remembers by Slurping Stuff From Its Environment
Slime mould might easily be one of the strangest life forms on our planet. They are neither plants, animals, nor fungi, but various species of complex, single-celled amoebas of the protist kingdom. Sometimes they form colonies able to grow, move, and

Even without a nervous system, they are able to learn about substances they encounter, retaining that knowledge and even communicating it to other slime moulds.

7 years ago
Spikes Of Graphene Can Kill Bacteria On Implants

Spikes of graphene can kill bacteria on implants

A tiny layer of graphene flakes becomes a deadly weapon and kills bacteria, stopping infections during procedures such as implant surgery

A tiny layer of graphene flakes becomes a deadly weapon and kills bacteria, stopping infections during procedures such as implant surgery. This is the findings of new research from Chalmers University of Technology, Sweden, recently published in the scientific journal Advanced Materials Interfaces.

Operations for surgical implants, such as hip and knee replacements or dental implants, have increased in recent years. However, in such procedures, there is always a risk of bacterial infection. In the worst case scenario, this can cause the implant to not attach to the skeleton, meaning it must be removed.

Bacteria travel around in fluids, such as blood, looking for a surface to cling on to. Once in place, they start to grow and propagate, forming a protective layer, known as a biofilm.

A research team at Chalmers has now shown that a layer of vertical graphene flakes forms a protective surface that makes it impossible for bacteria to attach. Instead, bacteria are sliced apart by the sharp graphene flakes and killed. Coating implants with a layer of graphene flakes can therefore help protect the patient against infection, eliminate the need for antibiotic treatment, and reduce the risk of implant rejection. The osseointegration – the process by which the bone structure grow to attach the implant – is not disturbed. In fact, the graphene has been shown to benefit the bone cells.

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7 years ago

Eye o' Sofia

Chasing the Shadow of Neptune’s Moon Triton

Our Flying Observatory

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Our flying observatory, called SOFIA, carries a 100-inch telescope inside a Boeing 747SP aircraft. Scientists onboard study the life cycle of stars, planets (including the atmosphere of Mars and Jupiter), nearby planetary systems, galaxies, black holes and complex molecules in space.

AND on Oct. 5, SOFIA is going on a special flight to chase the shadow of Neptune’s moon Triton as it crosses Earth’s surface!

In case you’re wondering, SOFIA stands for: Stratospheric Observatory for Infrared Astronomy.

Triton

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Triton is 1,680 miles (2,700 km) across, making it the largest of the 13 moons orbiting Neptune. Unlike most large moons in our solar system, Triton orbits in the opposite direction of Neptune, called a retrograde orbit. This backward orbit leads scientists to believe that Triton formed in an area past Neptune, called the Kuiper Belt, and was pulled into its orbit around Neptune by gravity. 

The Voyager 2 spacecraft flew past Neptune and Triton in 1989 and found that Triton’s atmosphere is made up of mostly nitrogen…but it has not been studied in nearly 16 years!

Occultations are Eclipse-Like Events

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An occultation occurs when an object, like a planet or a moon, passes in front of a star and completely blocks the light from that star. As the object blocks the star’s light, it casts a faint shadow on Earth’s surface. 

But unlike an eclipse, these shadows are not usually visible to the naked eye because the star and object are much smaller and not nearly as bright as our sun. Telescopes with special instruments can actually see these shadows and study the star’s light as it passes near and around the object – if they can be in the right place on Earth to catch the shadow.

Chasing Shadows

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Scientists have been making advanced observations of Triton and a background star. They’ve calculated exactly where Triton’s faint shadow will fall on Earth! Our SOFIA team has designed a flight path that will put SOFIA (the telescope and aircraft) exactly in the center of the shadow at the precise moment that Triton and the star will align. 

This is no easy feat because the shadow is moving at more than 53,000 mph while SOFIA flies at Mach 0.85 (652 mph), so we only have about two minutes to catch the shadow!! But our SOFIA team has previously harnessed the aircraft’s mobility to study Pluto from inside the center of its occultation shadow, and is ready to do it again to study Triton!

What We Learn From Inside the Shadow

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From inside the shadow, our team on SOFIA will study the star’s light as it passes around and through Triton’s atmosphere. This allows us to learn more about Triton’s atmosphere, including its temperature, pressure, density and composition! 

Our team will use this information to examine if Triton’s atmosphere has changed since our Voyager 2 spacecraft flew past it in 1989. That’s a lot of information from a bit of light inside a shadow! Similar observations of Uranus in 1977, from our previous flying observatory, led to the discovery of rings around that planet!

International Ground-Based Support

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Ground-based telescopes across the United States and Europe – from Scotland to the Canary Islands – will also be studying Triton’s occultation. Even though most of these telescopes will not be in the center of the shadow, the simultaneous observations, from different locations on Earth, will give us information about how Triton’s atmosphere varies across its latitudes. 

This data from across the Earth and from onboard SOFIA will help researchers understand how Triton’s atmosphere is distorted at different locations by its high winds and its strong tides!

Make sure to follow us on Tumblr for your regular dose of space: http://nasa.tumblr.com.

6 years ago

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7 years ago

Like the crazy eye of the universe

The Hydrogen Atom

The Hydrogen Atom

7 years ago

Biomimicry

Brittle Starfish Shows How To Make Tough Ceramics

Brittle starfish shows how to make tough ceramics

Nature inspires innovation. An international team lead by researchers at Technion – Israel Institute of Technology, together with ESRF -the European Synchrotron, Grenoble, France- scientists, have discovered how a brittle star can create material like tempered glass underwater. The findings are published in Science and may open new bio-inspired routes for toughening brittle ceramics in various applications that span from optical lenses to automotive turbochargers and even biomaterial implants.

A beautiful, brainless brittle star that lives in coral reefs has the clue to super tough glass. Hundreds of focal lenses are located on the arms of this creature, which is an echinoderm called Ophiocoma wendtii. These lenses, made of chalk, are powerful and accurate, and the deciphering of their crystalline and nanoscale structure has occupied Boaz Pokroy and his team, from the Technion-Israel Institute of Technology, for the past three years. Thanks to research done on three ESRF beamlines, ID22, ID13 and ID16B, among other laboratories, they have figured out the unique protective mechanism of highly resistant lenses.

As an example, take tempered glass. It is produced by exerting compressive pressure on the glass which compresses it and leaves it more compact than in its natural state. Glass tempering is performed by rapidly heating and then rapidly cooling the material. In this process, the outside of the material cools more quickly than the inside and thereby compresses the inside. Ophiocoma wendtiilenses are created in the open sea, at room temperature, unlike tempered glass. “We have discovered a strategy for making brittle material much more durable under natural conditions. It is ‘crystal engineering’ and tempering without heating and quenching – a process that could be very useful in materials engineering,” explains Pokroy.

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7 years ago

Researchers Find New DNA Structure in Living Human Cells

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Your daily selection of the latest science news!

According to Breaking Science News

A team of scientists from the Garvan Institute of Medical Research and the Universities of New South Wales and Sydney has identified a new DNA structure — called the intercalated motif (i-motif) — inside living human cells.

Deep inside the cells in our body lies our DNA. The information in the DNA code — all 6 billion A, C, G and T letters — provides precise instructions for how our bodies are built, and how they work.

The iconic ‘double helix’ shape of DNA has captured the public imagination since 1953, when James Watson and Francis Crick famously uncovered the structure of DNA.

However, it’s now known that short stretches of DNA can exist in other shapes, in the laboratory at least — and scientists suspect that these different shapes might play an important role in how and when the DNA code is ‘read.’

“When most of us think of DNA, we think of the double helix. This research reminds us that totally different DNA structures exist — and could well be important for our cells,” said co-lead author Dr. Daniel Christ, from the Kinghorn Centre for Clinical Genomics at the Garvan Institute of Medical Research and St Vincent’s Clinical School at the University of New South Wales.

“The i-motif is a four-stranded ‘knot’ of DNA,” added co-lead author Dr. Marcel Dinger, also from the Garvan Institute of Medical Research and the University of New South Wales.

“In the knot structure, C letters on the same strand of DNA bind to each other — so this is very different from a double helix, where ‘letters’ on opposite strands recognize each other, and where Cs bind to Gs [guanines].”

Although researchers have seen the i-motif before and have studied it in detail, it has only been witnessed in vitro — that is, under artificial conditions in the laboratory, and not inside cells. In fact, they have debated whether i-motif DNA structures would exist at all inside living things — a question that is resolved by the new findings.

To detect the i-motifs inside cells, Dr. Christ, Dr. Dinger and their colleagues developed a precise new tool — a fragment of an antibody molecule — that could specifically recognize and attach to i-motifs with a very high affinity.

Until now, the lack of an antibody that is specific for i-motifs has severely hampered the understanding of their role.

Crucially, the antibody fragment didn’t detect DNA in helical form, nor did it recognize ‘G-quadruplex structures’ (a structurally similar four-stranded DNA arrangement).

With the new tool, the team uncovered the location of ‘i-motifs’ in a range of human cell lines.

Using fluorescence techniques to pinpoint where the i-motifs were located, the study authors identified numerous spots of green within the nucleus, which indicate the position of i-motifs.

The scientists showed that i-motifs mostly form at a particular point in the cell’s ‘life cycle’ — the late G1 phase, when DNA is being actively ‘read.’

They also showed that i-motifs appear in some promoter regions — areas of DNA that control whether genes are switched on or off — and in telomeres, ‘end sections’ of chromosomes that are important in the aging process.

“We think the coming and going of the i-motifs is a clue to what they do. It seems likely that they are there to help switch genes on or off, and to affect whether a gene is actively read or not,” said study first author Dr. Mahdi Zeraati, also from the Garvan Institute of Medical Research and the University of New South Wales.

“We also think the transient nature of the i-motifs explains why they have been so very difficult to track down in cells until now,” Dr. Christ added.

“It’s exciting to uncover a whole new form of DNA in cells — and these findings will set the stage for a whole new push to understand what this new DNA shape is really for, and whether it will impact on health and disease,” Dr. Dinger said.

The team’s results appear in the journal Nature Chemistry.

Read more…

Got any news, tips or want to contact us directly? Email esistme@gmail.com

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This article and images were originally posted on [Breaking Science News] April 24, 2018 at 03:11PM. Credit to Author and Breaking Science News | ESIST.T>G>S Recommended Articles Of The Day

7 years ago
Organic Pigment Photocapacitors May Restore Sight To Blind People

Organic Pigment Photocapacitors May Restore Sight to Blind People

A simple retinal prosthesis is being developed in collaboration between Tel Aviv University in Israel and LiU. Fabricated using cheap and widely-available organic pigments used in printing inks and cosmetics, it consists of tiny pixels like a digital camera sensor on a nanometric scale. Researchers hope that it can restore sight to blind people.

Researchers led by Eric Glowacki, principal investigator of the organic nanocrystals subgroup in the Laboratory of Organic Electronics, Linköping University, have developed a tiny, simple photoactive film that converts light impulses into electrical signals. These signals in turn stimulate neurons (nerve cells). The research group has chosen to focus on a particularly pressing application, artificial retinas that may in the future restore sight to blind people. The Swedish team, specializing in nanomaterials and electronic devices, worked together with researchers in Israel, Italy and Austria to optimise the technology. Experiments in vision restoration were carried out by the group of Yael Hanein at Tel Aviv University in Israel. Yael Hanein’s group is a world-leader in the interface between electronics and the nervous system.

The results have recently been published in the prestigious scientific journal Advanced Materials.

Read more.

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